Abstract:Ball milling pretreatment can be used to change the physical and chemical structure of corn straw, making it much easier to be degraded by enzymes, which will enhance the microorganism utilization rate to improve its feed quality. This study indicated that the particle size of corn straw after ball milling mainly(90%) distributed in the range of 10~100 μm and ball mill crushed corn straw had a 3.5% decrease in Acid Detergent Fiber(ADF) content and a 200% increase in soluble sugar content due to the breaking of hemicellulose chain and cellulose chain. The total content of soluble sugar and reducing sugar of ball milling corn straw hydrolysed by xylanase and cellulase increased by 3.3 fold and 9.7 fold than that without treatment, separately. The total acid content reached 26.3 mg/g and the lactic acid bacteria population was about 1.5×109 CFU/g after corn straw solid state fermentation. Furthermore, the cellulose and hemicellulose separately decreased by 9.1 and 4.9 percentage points than before, which could significantly improve the quality of the feed.