Breeding of High-Yield L-Serine Producing Strains Which Can Ferment Sugar Directly

doi:10.3969/j.issn.1673-1689.2014.11.011

Home > Archive>Volume 33, Issue 11, 2014 >1191-1195. DOI:10.3969/j.issn.1673-1689.2014.11.011

Breeding of High-Yield L-Serine Producing Strains Which Can Ferment Sugar Directly
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                        10.3969/j.issn.1673-1689.2014.11.011
                    
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C. glutamicum SYPS-062-36 could produce L-serine from sugar directly, in order to further improve L-serine production via removing the feedback inhibition of PGDH caused by high concentration of L-serine, a mutant strain C. glutamicum SYPS-062-33a-18 was obtained by DES treatment of C. glutamicum SYPS-062-36 for two rounds and selecting positive mutant in the plate containing D-serine. The genetic stable mutant strain could accumulate 11.0 ± 0.25 g/L of L-serine, with an increase of 65.4% compared with the parental strain. These results suggested that L-serine overproduced strain can be obtained by using random mutagenesis and directed selection with D-serine as the analog, and also provide a valuable basis for further improving L-serine production with inverse metabolic engineering.

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XU Guoqiang, DOU Wenfang, XU Hongyu, ZHANG Xiaomei. Breeding of High-Yield L-Serine Producing Strains Which Can Ferment Sugar Directly[J]. Journal of Food Science and Biotechnology,2014,33(11):1191-1195.

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Online: December 19,2014
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