Abstract:Croton fruits(Euphorbiaceae) has been applied for the treatment of some types of cancer in traditional Chinese medicine for a long time. Lingba Junzhi is a fermented product of croton fruits by Ganoderma lucidum(Curtis：Fr.) P. Karst. The paper was fixed to screen the effective fractions about resisting tumor in Lingba Junzhi in vitro. Soxhlet extraction was used to extract Lingba Junzhi with the gradient solvents, and four extract fractions were obtained, petroleum ether extracts(PEE), dichloromethane extracts, normal butanol extracts and methanol extracts. Then methanol extracts were dealt with the alkali-solution and acid-isolation and solvent extraction, to prepare alkaline methanol extracts(AME). Screening anti-tumor effects of PEE and AME to SMMC-7721, MGC-803, A549, HepG-2 tumor cells were investigated by means of quick and sensitive MTT colorimetry. Compraring with crotonoside(Cro), PEE and AME inhibited the proliferation of A549 cells significantly in a dose-dependent manner, and had nearly no influence on MGC-803 cells. Within a certain concentration range, AME played a strong inhibited effect only to the SMMC-7721 proliferation, while PEE and AME worked for the promotion of the HepG-2 proliferation. All these mentioned above suggest that the effects on proliferative activity of different parts of Lingba Junzhi is quite different. But PEE and AME all have the obvious anti-tumor effects on A549 cell proliferation.
