Abstract:The aim of this study is to develop MAE-GC/MS method to determine the Benzo(α) pyrene (B(α)P) content. For this, the sample was pre-treatment by MAE(microwave-assisted extraction technology)and then separate by, DB-5MS fused silica capillary column(30 m×0.25 mm×0.25 μm) with B(α)P m/z=252 asthe selected ion, the detection is the series four quadrupole selected ion monitoring(SIM) mode with 9-phenyl anthracene as internal standard. A series of results were achieved： B(α)P detection limit was 0.01 μg/kg and the standard curve was linear at the range of 0~10 μg/mL. The correlation coefficient was 1.000, with the recoveries of 97%~103% and good precision: Days RSD/%<0.5%, day RSD/%<1%. The good accuracy, the negative sample as the base material, containing 0.1 mL methanol and benzo (α) pyrene national standard component analysis (GBW08702) spiked levels, the measured values within the standard value range. Based on the above results, conclusion could be arrived: the method is simple, high recovery, detection limit, linear range, accuracy, precision results meet the requirements for the determination of food in B(α)P content.
