New Method of Electrochemical Immune Detection of Immunoglobulin G

doi:10.3969/j.issn.1673-1689.2015.01.008

Home > Archive>Volume 34, Issue 1, 2015 >47-53. DOI:10.3969/j.issn.1673-1689.2015.01.008

New Method of Electrochemical Immune Detection of Immunoglobulin G
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                        10.3969/j.issn.1673-1689.2015.01.008
                    
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To develop a novel electrochemical immune biosensor based on antigen-antibody reaction with the immunoglobulin G immobilized the surface of the ITO. The side of ITO was immobilized the goat anti-human IgG after the amino-modified and glutaraldehyde modification. The side of SiO2 also had the same modification of amino and Glutaraldehyde, and then 2~5 nm gold nanoparticles adsorbing the surface of SiO2 by the use of electrostatic interaction. We immobilized the goat anti-human IgG after the reduction of H2O2 with bigger gold nanoparticles. When human IgG respectively combined with the ITO and nanomaterials, it would form the sandwich structure of a new detection of immunoglobulin G based on Au/SiO2 to amplify signal amplification. The prepared biosensor showed a excellent linearity relationship of Cyclic voltammograms current value and the concentration of immunoglobulin G 1~200 ng/mL, the equation of linear regression is y=-0.532 6x+409.256 7（R2=0.994 8）, with a detection limit of 0.6 ng/mL. The established method is low cost and ultrasensitive, and can be used to detect immunoglobulin G.

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NI Lili, SONG Liangjing, MA Xiaoyuan, WU Shijia, DUAN Nuo, WANG Zhouping. New Method of Electrochemical Immune Detection of Immunoglobulin G[J]. Journal of Food Science and Biotechnology,2015,34(1):47-53.

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Online: February 06,2015
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