Study on High Level Expression of Cetuximab in Vitro Culture by Different Ventilationand Stirring Conditions in CHO Cells

doi:10.3969/j.issn.1673-1689.2015.01.012

Home > Archive>Volume 34, Issue 1, 2015 >74-78. DOI:10.3969/j.issn.1673-1689.2015.01.012

Study on High Level Expression of Cetuximab in Vitro Culture by Different Ventilationand Stirring Conditions in CHO Cells
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                        10.3969/j.issn.1673-1689.2015.01.012
                    
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Four different Ventilation and stirring conditions were established , Taking CHO cells expressed Cetuximab as research object and adopting the serum-free mediumand suspension culture, the effect of different Ventilation conditions on cell density、cell viability and protein concentration were contrasted. The results show that compared with traditional Ventilation like micro bubble and no bubble, cell density and protein concentration of big bubble Ventilation was almost the same even better in some respects. The new aeration not only changed the microenvironment of the CHO cells, but also had a great advantage in the cleaning validation. This Study laid a foundation for the application of big bubble ventilation on Large-scale Mammalian Cell Culture, and also laid a foundation for the optimization of CHO culture technology in pilot scale.

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WENG Zhibing, SHAO Chunhua, HU Hui. Study on High Level Expression of Cetuximab in Vitro Culture by Different Ventilationand Stirring Conditions in CHO Cells[J]. Journal of Food Science and Biotechnology,2015,34(1):74-78.

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Online: February 06,2015
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