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Home > Archive>Volume 35, Issue 1, 2016 >12-18. DOI:10.3969/j.issn.1673-1689.2016.01.002
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Enhanced Production of Solvent-Stable Alpha Glycosidase from Bacillus licheniformis JXC-1 by an Optimal Feeding Strategy
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    Abstract:

    In previous work，we screened a strain（Bacillus licheniformis JXC-1） producing solvent-stable（10% N，N-Dimethylformamide，short for DMF） α-glucosidase from several different soil samples. In this work，we attempted to determine the key factors for the production of α-glucosidase and optimize the feeding strategy in a 3-L fermenter. Results showed that the enzyme activity reached 444.7 U/L with the optimal condition（pH 7.0，the initial maltose concentration 25 g/L，and agitation speed 600 rpm）. The strategy of feeding maltose and tryptone at a rate of 2.25 mL/h（4-5 h），6.75 mL/h（5-6 h），9 mL/h（6-7 h） and 15 mL/h（7-8 h） significantly increased the production of α-glycosidase from 444.7 U/L to 872.5 U/L.

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TANG Qunfang, LI Jianghua, LIU Long, DU Guocheng. Enhanced Production of Solvent-Stable Alpha Glycosidase from Bacillus licheniformis JXC-1 by an Optimal Feeding Strategy[J]. Journal of Food Science and Biotechnology,2016,35(1):12-18.

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  • Online: March 11,2016
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