Treatment of Organic Solvents to Improve Activity and Stability of Immobilized Lipases

doi:10.3969/j.issn.1673-1689.2016.01.014

Home > Archive>Volume 35, Issue 1, 2016 >89-94. DOI:10.3969/j.issn.1673-1689.2016.01.014

Treatment of Organic Solvents to Improve Activity and Stability of Immobilized Lipases
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                        10.3969/j.issn.1673-1689.2016.01.014
                    
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The lipases immobilized on active carbon，silica gel G or DM-130 macro porous resin by adsorption were steeped in various organic solvents to improve the activity and stability for catalyzing transesterification. It was found that the activities of immobilized lipases could be increased with acetone，1-propanol，2-propanol and ethyl acetate，but could be deactivated with methanol，n-hexane and cyclohexane. Compared with the immobilized lipases dried directly after adsorption，the immobilized lipases treated in 2-propanol or ethyl acetate for 1 h displayed 1.70 to 2.25 times enzyme activities，their half-lives were prolonged 3.2 to 8.4 h and the operation lives were increased 2 to 5 batches for soybean oil transesterification. The thermal stability of the immobilized lipases could also be enhanced by the treatment with 2-propanol or ethyl acetate.

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ZU Xin, DU Kai, CAO Fang. Treatment of Organic Solvents to Improve Activity and Stability of Immobilized Lipases[J]. Journal of Food Science and Biotechnology,2016,35(1):89-94.

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Online: March 11,2016
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