Sandwich Immunoassy Against Pseudomonas syringae pv. Maculicola

doi:10.3969/j.issn.1673-1689.2016.02.008

Home > Archive>Volume 35, Issue 2, 2016 >162-165. DOI:10.3969/j.issn.1673-1689.2016.02.008

Sandwich Immunoassy Against Pseudomonas syringae pv. Maculicola
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                        10.3969/j.issn.1673-1689.2016.02.008
                    
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Abstract:

A sandwich immnuoassay based on monoclonal antibody for detection of Pseudomonas syringae pv. Maculicola was developed. Killed Pseudomonas syringae pv. Maculicola cell was used as immunogen to immunize BALB/c mice. The antisera was detected and the mice of highest antisera titer was selected for cell fusion. With hybirdoma technology，six cell lines secreting specific antibody to Pseudomonas syringae pv. Maculicola were obtained. Monoclonal antibodies （mAb） coded mAb1 and mAb6 were selected used as coating antibody and detection antibody based on pair-matching screening results. The sandiwich measure based on double antibodies were found to be high specific to targeted bacteria and no cross reactions to other bacteria including Burkholderia glumae，Xanthomonas oryzae pv. Oryzicola，Pantoea stewartii subsp.stewartii and Pseudomonas syringae pv.syringae. Under optimized conditions，the limit of detection was 1.5×105 cfu/mL and quantitative limit was 4.12×105 cfu/mL. This immunoassy was highly specific to Pseudomonas syringae pv. Maculicola and suitable for in-field detction，which provide a convient and fast tool in plant bacteria analysis.

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WANG Wenbin, KONG Dezhao, TANG Lijuan, MA Wei. Sandwich Immunoassy Against Pseudomonas syringae pv. Maculicola[J]. Journal of Food Science and Biotechnology,2016,35(2):162-165.

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Online: October 25,2016
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