Co-Production of L-Arginine and Polyhydroxybutyrate in Recombinant Corynebacterium crenatum

doi:10.3969/j.issn.1673-1689.2016.03.003

Home > Archive>Volume 35, Issue 3, 2016 >240-246. DOI:10.3969/j.issn.1673-1689.2016.03.003

Co-Production of L-Arginine and Polyhydroxybutyrate in Recombinant Corynebacterium crenatum
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                        10.3969/j.issn.1673-1689.2016.03.003
                    
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Corynebacterium crenatum SYPA 5-5 is a industrial strain for L-arginine production. Polyhydroxybutyrate （PHB）is a kind of polymer stored in the bacterial as carbon source and energy. Due to its environment-friendly properties such as biodegradability and biocompatibility，PHB can be made into plastic containers and surgical suture to reduce the white pollution and can be digested easily in the body. It was reported that co-producing PHB and some other industrial valuable compounds，such as succinate，L-glutamate and tryptophan，were possible by introducing PHB metabolic pathways into the cells to influence the intracellular pathways. In this study，a co-produce PHB and L-arginine strain was constructed by introducing the entire phbCAB operon of Ralstonia eutropha H16 into C. crenatum SYPA 5-5. Fermentation were conducted of the start strain and recombinant one，separately，in a 5-L fermentor and relevant fermentation parameters were detected and analysed. The result showed that intracellular PHB（12.8% of DCW） and extracellular L-arginine （43.21 g/L） were obtained and accumulated in the recombinant strain，.In addition，The PHB production also promote the L-arginine yield，which weas increased by 20%.

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QIN Jingru, XU Meijuan, ZHANG Xian, YANG Taowei. Co-Production of L-Arginine and Polyhydroxybutyrate in Recombinant Corynebacterium crenatum[J]. Journal of Food Science and Biotechnology,2016,35(3):240-246.

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