Improved 2,3-Butanediol Production by Overexpressing Acetoin Reductase in Bacillus subtilis 168

doi:10.3969/j.issn.1673-1689.2016.03.005

Home > Archive>Volume 35, Issue 3, 2016 >252-257. DOI:10.3969/j.issn.1673-1689.2016.03.005

Improved 2,3-Butanediol Production by Overexpressing Acetoin Reductase in Bacillus subtilis 168
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                        10.3969/j.issn.1673-1689.2016.03.005
                    
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Bacillus subtilis 168 is a safe strain for industrial-scale microbial production of 2，3-butanediol （2，3-BD）. However， a large quantity of by-product of acetoin （AC） is accumulated during 2，3-BD fermentation process. acetoin reductase （ACR） is the key enzyme which catalyzes the conversion of acetoin to 2，3-BD. In this study，in order to improve 2，3-BD production，we firstly cloned the acr gene of acetoin reductase into B. subtilis 168，and then further constructed the recombinant strain B. subtilis 168/pMA5-acr. The results showed that the yield and conversion rate of the 2，3-BD were increased up to 28.62% and 22.87% by the recombinant strain，respectively. Whereas the accumulation of the main by-product of acetoin decreased by 20.01%. Furthermore，the molar yields of by-products of formic acid，acetic acid，lactic acid，and succinate were also decreased.

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LI Xiupeng, YANG Taowei, XU Meijuan, ZHANG Xian. Improved 2,3-Butanediol Production by Overexpressing Acetoin Reductase in Bacillus subtilis 168[J]. Journal of Food Science and Biotechnology,2016,35(3):252-257.

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