Efficient Preparation of (R)-2-Chloro-1-(3-Chlorophenyl) Ethanol by a Double Enzyme System

doi:10.3969/j.issn.1673-1689.2016.03.009

Home > Archive>Volume 35, Issue 3, 2016 >278-283. DOI:10.3969/j.issn.1673-1689.2016.03.009

Efficient Preparation of (R)-2-Chloro-1-(3-Chlorophenyl) Ethanol by a Double Enzyme System
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                        10.3969/j.issn.1673-1689.2016.03.009
                    
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A codon-optimized gene Sys1，which encodes a carbonyl reductase，was synthesized，and cloned into an expression plasmid pETDuet-1 with double promoters together with a glucose dehydrogenase-encoding gene Sygdh. The recombinant E. coli BL21/pETDuet-Sygdh-Sys1，expressing the carbonyl reductase and the glucose dehydrogenase together，was constructed by transforming the recombinant plasmid，pETDue-Sygdh-Sys1，into E. coli BL21（DE3）. Using recombinant E. coli cells induced by IPTG as biocatalysts，（R）-2-chloro-1-（3-chlorophenyl）ethanol（（R）-CCE），a chiral drug intermediate，was synthesized by asymmetrically reducing m-chlorophenacyl chloride（m-CPC）. Under the reaction conditions of 30 mmol/L of m-CPC，70 mg/mL of wet recombinant E. coli cells，40 mmol/L of glucose，0.2 mmol/L of NADP+ at pH 7.0 and 40 ℃ for 3 h，the molar yield of（R）-CCE reached 99.0% with an enantiomeric excess（e.e.） value of 100%.

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ZHU Lijuan, YU Tao, GU Ying, YANG Biao, WU Minchen. Efficient Preparation of (R)-2-Chloro-1-(3-Chlorophenyl) Ethanol by a Double Enzyme System[J]. Journal of Food Science and Biotechnology,2016,35(3):278-283.

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