Study of High Cell-Density Fermentation of Laccase Produced by Pichia pastoris X33/pGAPZαA-MLcc1

doi:10.3969/j.issn.1673-1689.2016.03.016

Home > Archive>Volume 35, Issue 3, 2016 >324-329. DOI:10.3969/j.issn.1673-1689.2016.03.016

Study of High Cell-Density Fermentation of Laccase Produced by Pichia pastoris X33/pGAPZαA-MLcc1
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                        10.3969/j.issn.1673-1689.2016.03.016
                    
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The expression of laccase gene by Pichia pastoris X33/pGAPZα A-MLcc1 was investigated in a 5 L fermenter. The change trend of laccase activity was consistent with the change trend dry cell weight. The extracellular laccase activity reached the highest value of 47.2 U/L when the yeast X33 entered the stationary phage at 46 h. High-density fermentation in a 5 L fermenter was further investigated and the optimized method for high-level expression was obtained as follows：by adopting dissolved oxygen（DO） feed-back feeding strategy and maintaining DO at 15% to 30% at feeding stage，as well as adding ammonia solution and hydrochloride to maintain pH value at 5 in the fermentation stage，the laccase activity reached 216.3 U/L，which was 4.6 times higher than that of fed batch fermentation.

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HUANG Liang, LIU Yihan, LIU Xiaoguang, LI Yu, LU Fuping. Study of High Cell-Density Fermentation of Laccase Produced by Pichia pastoris X33/pGAPZαA-MLcc1[J]. Journal of Food Science and Biotechnology,2016,35(3):324-329.

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