Identification of Three Rice Pathogens by MALDI-TOF-MS

doi:10.3969/j.issn.1673-1689.2016.04.006

Home > Archive>Volume 35, Issue 4, 2016 >370-374. DOI:10.3969/j.issn.1673-1689.2016.04.006

Identification of Three Rice Pathogens by MALDI-TOF-MS
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                        10.3969/j.issn.1673-1689.2016.04.006
                    
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Abstract:

After Burkholderia glumae (NCPPB 2391, NCPPB 3591), Xanthomonas oryzae pv. Oryzicola (NCPPB 1585) and Pseudomonas syringae pv. Syringae (NCPPB2844) were cultured by Columbia agar medium and bacterial colonies were treated with an ethanol-formic acid extraction procedure, the protein fingerprints were obtained and analyzed by MALDI-TOF-MS. Three plant pathogens were accurately identified except that Pseudomonas syringae pv. Syringae did not exist in the Bio-Typer. With the standard method from Bruker, we established the protein fingerprint of Pseudomonas syringae pv. syringae in Bio-Typer. For the rice leaf spiked with these rice pathogens,

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WANG Wenbin, KUANG Hua, XU Liguang, MA Wei, LIU Liqiang, XU Chuanlai. Identification of Three Rice Pathogens by MALDI-TOF-MS[J]. Journal of Food Science and Biotechnology,2016,35(4):370-374.

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