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Home > Archive>Volume 35, Issue 4, 2016 >419-423. DOI:10.3969/j.issn.1673-1689.2016.04.013
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A DPO- PCR Method for the Rapid Detection of Staphylococcus aureus
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    Abstract:

    A dual-priming oligonucleotide (DPO)-based PCR was developed for the rapid detection of Staphylococcus aureus (SA) using Sa442 of SA as a target gene. DPO primers were able to efficiently amplify the target gene in a temperature range from 45 ℃ to 65 ℃，indicating that the DPO-PCR method was not sensitive to the annealing temperature. The DPO-PCR sensitivity was 2.27×102 cfu/mL. Because of a high specificity of DPO-PCR due to the special structures of DPO primers，no non-specific amplifications were produced in reaction. In addition，13 positive samples for SA were detected from 175 clinical samples by the DPO-PCR method，which was in accordance with the result by the SN/T 1870-2007 standard detection protocol. Therefore，the DPO-PCR could be used as a sensitive，rapid and simple tool.

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LI Dandan, XU Yigang, QIU Suoping, WANG Yu, GAO Huijiang, GAO Shenyang. A DPO- PCR Method for the Rapid Detection of Staphylococcus aureus[J]. Journal of Food Science and Biotechnology,2016,35(4):419-423.

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  • Online: November 01,2016
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