Fermentation Optimization of Chymosin Production by Recombinant Pichia pastoris

doi:10.3969/j.issn.1673-1689.2016.05.002

Home > Archive>Volume 35, Issue 5, 2016 >457-464. DOI:10.3969/j.issn.1673-1689.2016.05.002

Fermentation Optimization of Chymosin Production by Recombinant Pichia pastoris
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                        10.3969/j.issn.1673-1689.2016.05.002
                    
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In order to obtain the optimal condition of chymosin fermentation，we observed the influence of initial inducing OD600，methanol concentration and pH on the high density fermentation process in 3.6 L fermentor of P. pastoris GS115/pPIC9k-chy. By using glycerol feeding strategy with DO control in high-density fermentation and maintained the residual methanol concentration constantly with a methanol sensor. The result showed that the optimum condition were listed as follows：the OD600 was 150，the methanol concentration was 2.0% and the pH was 5.0，then the highest chymosin activity（17 80 SU/mL），the highest chymosin productivity（19.48 SU/（mL·h）） and the highest yield of products（2.42 SU/mL）were achieved，the total protein content and the ratio of milk-clotting activity to proteolytic activity（MCA/PA） were determined to be 0.64 mg/mL，63.43.

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LI Liang, WU Dan, SU Lingqia, DUAN Xuguo, WU Jing. Fermentation Optimization of Chymosin Production by Recombinant Pichia pastoris[J]. Journal of Food Science and Biotechnology,2016,35(5):457-464.

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