Screening of High L-Asparaginase Activity Mutants of Recombinant Bacteria WB600 by Atmospheric and Room Temperature Plasmas Mutation System

doi:10.3969/j.issn.1673-1689.2016.05.006

Home > Archive>Volume 35, Issue 5, 2016 >485-491. DOI:10.3969/j.issn.1673-1689.2016.05.006

Screening of High L-Asparaginase Activity Mutants of Recombinant Bacteria WB600 by Atmospheric and Room Temperature Plasmas Mutation System
DOI:
                        10.3969/j.issn.1673-1689.2016.05.006
                    
CSTR:
                        [cstr]
                    
Author:
                        
                        
                    
Affiliation:
Clc Number:
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

L-asparaginase（L-ASNase） can catalyze the deamidation of L-asparagine（a precursor of toxic acrylamide） to L-aspartic acid and ammonia，and it is thus an important food enzyme. This study used Bacillus subtilis WB600 as the expression host，successfully realizing the secretory expression of L-ASNase. By using the atmospheric and room temperature plasmas（ARTP） mutation system，the titer of the recombinant L-ASNase was improved further. After 30 h fermentation，extracellular enzyme activity of recombinant B. subtilis WB600（pMA5- wapA -ansZ）reached 37.2 U/mL. This result suggested that L-ASNase can secrete to the medium mediated by a signal peptide of wapA. When treated with the ARTP at power input 120 W and gas flow rate 10 L/min for 40 s，a mutant of B. subtilis WB600（pMA5- wapA -ansZ）with a activity of 48.4 U/mL was obtained，the activity value was increased by 30%. These results suggested that ARTP mutation system can be used to improve the production of L-ASNase of recombinant strains. This study provided a good L-ASNase producing strain to fermentation production at an industrial scale.

Reference

Cited by

Get Citation

CHEN Xuan, LIU Song, FENG Yue, DU Guocheng, CHEN Jian. Screening of High L-Asparaginase Activity Mutants of Recombinant Bacteria WB600 by Atmospheric and Room Temperature Plasmas Mutation System[J]. Journal of Food Science and Biotechnology,2016,35(5):485-491.

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:
Revised:
Adopted:
Online: November 01,2016
Published:

Article QR Code

Copy Right：Editorial Board of Journal of Food Science and Biotechnology

Address：No. 1800, Lihu Avenue, Wuxi 214122, Jiangsu Province,China PostCode：214122

Phone：0510-85913526 E-mail：xbbjb@jiangnan.edu.cn

Supported by:Beijing E-Tiller Technology Development Co., Ltd.

Get Citation

Share

Article Metrics

History

Article QR Code

WeChat

Mobile website