Construction of Secretory Escherichia coli Expression System of Malolactic Enzyme

doi:10.3969/j.issn.1673-1689.2016.05.008

Home > Archive>Volume 35, Issue 5, 2016 >498-503. DOI:10.3969/j.issn.1673-1689.2016.05.008

Construction of Secretory Escherichia coli Expression System of Malolactic Enzyme
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                        10.3969/j.issn.1673-1689.2016.05.008
                    
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Malolactic enzyme（MLE） is a key enzyme inmalactic fermentation（MLF）during fruit-wine producing. In order to construct the secretory Escherichia coli（E.coli）expression system of MLE. The signal peptide gene（487 bp） and the MLE gene（1 623 bp） were combined through fusion-PCR，then the fused fragment was inserted into pET28a（+） and transformed into E.coil. 60 000 protein was obtained when the positive strain was cultured and induced with IPTG. 240.7 mg/L L-lactic acids could be detected in the fermentation supernatant with HPLC. All the results indicated the secretory E.coli expression system of MLE was successfully constructed. This will provide a technical platform for the research and application of MLE.

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WANG Ningning, LIU Rui, CHEN Fusheng, ZHANG Xiuyan. Construction of Secretory Escherichia coli Expression System of Malolactic Enzyme[J]. Journal of Food Science and Biotechnology,2016,35(5):498-503.

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Online: November 01,2016
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