Sequence Variation Regularity Analysis of 16S rRNA Gene of Vibrio parahaemolyticus

doi:10.3979//j.issn.1673-1689.2016.09.005

Home > Archive>Volume 35, Issue 9, 2016 >928-934. DOI:10.3979//j.issn.1673-1689.2016.09.005

Sequence Variation Regularity Analysis of 16S rRNA Gene of Vibrio parahaemolyticus
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                        10.3979//j.issn.1673-1689.2016.09.005
                    
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In order to lay a foundation for the development of new targets for typing and tracing，we investigated the characteristics of the variations based on the analysis of 16S rRNA gene sequences of Vibrio parahaemolyticus.We sequenced 16S rRNA gene of 111 strains of Vibrio parahaemolyticus which were isolated from fresh aquatic products，and aligned with standard sequence in GenBank. We also established phylogenetic tree of sequences which had a great variation，and verified the variation regularity of the genes by ERIC-PCR.The variability of different Vibrio parahaemolyticus of 16S rRNA genes was discovered. The variation mainly centralized in variable region V1 and V2. There were 38 base sites which similarity were less than 30% in the region of 50~270 bp，and 103 base sites which similarity ranged from 30% to 100%. In addition，there was one base which showed difference both at V3 and V8. The variability of different Vibrio parahaemolyticus of 16S rRNA genes can lay a foundation for the development of new targets for typing and tracing of Vibrio parahaemolyticus and for the study of micro-evolution.

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FENG Bo, LAN Ying, SUN Xiaohong, ZHANG Weijia, PAN Yingjie, ZHAO Yong. Sequence Variation Regularity Analysis of 16S rRNA Gene of Vibrio parahaemolyticus[J]. Journal of Food Science and Biotechnology,2016,35(9):928-934.

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