Optimization of Subcritical Water Extraction of Polysaccharides from Auricularia auricular by Response Surface Methodology
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    Abstract:

    The optimization of subcritical water extraction of polysaccharides from Auricularia auricular was investigated by response surface methodology (RSM). The effect of extraction condition on the yield of polysaccharies in Auricularia auricular powder was studied,including temperature,extraction duration,liquid-solid ratio and pressure. The content of polysaccharide was determined using phenol-sulfuric acid colorimetry. A Box-Behnken design was used to achieve the best extraction procedure based on the results of single-factor experiments. The comparison of different extraction methods was also done. The optimized extraction procedure was performed by 131∶1 of liquid-solid ratio at 152 ℃ for 26 min under the pressure of 1.0 MPa with the polysacchariesn yield of 24.51%. The yield of polysacchariesn was significantly increased using the proposed subcritical water extraction procedure,which was 3.82 fold of that extracted by traditional hot water method. This study provides a high-efficiency and eco-friendly method for industrial production of polysaccharides from Auricularia auricular.

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BAO Yihong, DENG Qi. Optimization of Subcritical Water Extraction of Polysaccharides from Auricularia auricular by Response Surface Methodology[J]. Journal of Food Science and Biotechnology,2016,35(10):1053-1060.

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  • Online: October 31,2016
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