Detection for Sika Deer-Origin Ingredients in Foods by Fluorescent Real-Time PCR Method

doi:10.3969/j.issn.1673-1689.2016.10.012

Home > Archive>Volume 35, Issue 10, 2016 >1088-1092. DOI:10.3969/j.issn.1673-1689.2016.10.012

Detection for Sika Deer-Origin Ingredients in Foods by Fluorescent Real-Time PCR Method
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                        10.3969/j.issn.1673-1689.2016.10.012
                    
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A method based on fluorescent real-time polymerase chain reaction was developed to detect sika deer-ingredient in food products. By comparative analysis of sika deer gene sequences，sensitive primers and TaqMan probe were designed on a conserved region of mitochondrial cytochrome c oxidase subunit I sequence，using the Primer Express，Oligo and DNAMAN software. Furthermore，the species specificity and detection sensitivity were tested and validated by using standard samples. Results indicated that primers and TaqMan probe have a high sensitivity for sika deer without non-targeted amplification for other species DNA. Sensitivity of detection was confirmed to be 0.42 pg per reaction with a 91.68% amplification efficiency. Real-time PCR method was applied to detect sika deer meat in the market. Compared with the standard method，this method was more rapid，accurate and convenient，which was suitable for fast authentication of sika deer-origin ingredients.

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QI Chunmeng, YANG Xinting, XUE Chenyu, ZHAO Linna, HOU Caiyun. Detection for Sika Deer-Origin Ingredients in Foods by Fluorescent Real-Time PCR Method[J]. Journal of Food Science and Biotechnology,2016,35(10):1088-1092.

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Online: October 31,2016
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