Enhanced Expression of Hyalurondiase in Pichia pastoris by Two-Stage Temperature Induction Strategy

doi:10.3969/j.issn.1673-1689.2016.12.007

Home > Archive>Volume 35, Issue 12, 2016 >1273-1277. DOI:10.3969/j.issn.1673-1689.2016.12.007

Enhanced Expression of Hyalurondiase in Pichia pastoris by Two-Stage Temperature Induction Strategy
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                        10.3969/j.issn.1673-1689.2016.12.007
                    
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Hyalurondiases（HAase） are a kind of hydrolases with medicine values. In order to improve the expression of HAase in the recombinant Pichia pastoris，the effect of induction temperature on HAase expressed was investigated in the recombinant P. pastoris. The result showed that the production of HAase could be improved with the decrease of induction temperatures，especially，two-stage temperature induction strategy. In this strategy，temperature was controlled at 25 ℃ during the induction stage 0~60 h，and then shifted to 22 ℃ after 60 h. HAase activity reached to 1.18×106 U/mL，2.6 fold improvement compared to induction temperature at 30 ℃（4.53×105 U/mL）. In addition，two-stage temperature induction strategy had an advantage on cell viability and alcohol oxidase activity，which offered a new strategy for expression of exogenous protein in the recombinant P. pastoris.

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ZHANG Na, LI Jianghua, JIN Peng, DU Guocheng, CHEN Jian, KANG Zhen. Enhanced Expression of Hyalurondiase in Pichia pastoris by Two-Stage Temperature Induction Strategy[J]. Journal of Food Science and Biotechnology,2016,35(12):1273-1277.

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