Rapid Detection of Salmonellla spp. in Edible Fungi by Droplet Digital PCR

doi:10.3969/j.issn.1673-1689.2017.03.014

Home > Archive>Volume 36, Issue 3, 2017 >315-321. DOI:10.3969/j.issn.1673-1689.2017.03.014

Rapid Detection of Salmonellla spp. in Edible Fungi by Droplet Digital PCR
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                        10.3969/j.issn.1673-1689.2017.03.014
                    
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Quantitative Risk Assessment（QRA） is the future trend of the risk assessment system in which simple，rapid and accurate quantitative technologies made crucial contributions to the smooth and efficient accomplishment of QRA. In this study，a method for detecting the virulence-specific gene，invA in Salmonellla spp. was established by using droplet digital PCR technology. In contrast to the traditional method of national standard（GB 4789.4-2010） for Salmonellla detection，our developed method showed higher specificity and sensitivity as low as 102 CFU/mL. The coincidence rate was 100 % as compared with simulation specimens with culture strains. The establishment of this method based on droplet digital PCR for Salmonella detection provided a rapid and accurate approach for identification of food-borne pathogens without the use of standard curves or plasmids. These results demonstrated that this developed method offered a promising way for rapid quantitative detection of Salmonella and provided technical support for the future studies of the microbial risk assessment system.

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ZHAO Xin, LAN Qingkuo, CHEN Rui, ZHU Zhu, LIU Na, WANG Yong. Rapid Detection of Salmonellla spp. in Edible Fungi by Droplet Digital PCR[J]. Journal of Food Science and Biotechnology,2017,36(3):315-321.

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Online: April 28,2017
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