Anti-Tumor Proliferation Activity of hIL-29 Variant

doi:10.3969/j.issn.1673-1689.2017.05.018

Home > Archive>Volume 36, Issue 5, 2017 >553-559. DOI:10.3969/j.issn.1673-1689.2017.05.018

Anti-Tumor Proliferation Activity of hIL-29 Variant
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                        10.3969/j.issn.1673-1689.2017.05.018
                    
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In order to analyze the antineoplastic activity of human interleukin-29（hIL-29） variant，a mutant gene of hIL-29 was amplified by megaprimer PCR，and the Arg35 of hIL-29 peptide chain was mutated to Lys35. A recombinant plasmid was constructed and transformed into Pichia pastoris GS115，the recombinant protein rhIL-29mut35 was induced and purified. The purified rhIL-29mut35 had a relative molecular mass of about 23×103 on SDS-PAGE profile and showed specific reaction with goat anti-human IL-29 polyclonal antibody in Western blotting. With the Cell Counting Kit-8 reagent，the rhIL-29mut35 was found to has anti-proliferation effect on tumor cells SGC7901，BEL7402，A549 and HCT8 separately with inhibition ratios of （34.20±1.50）%，（27.30±0.31）%，（30.20±0.68）% and（29.13±1.40）% in the high dose group. The anti-proliferation effect of rhIL-29mut35 was stronger than that of wild type rhIL-29（P<0.01），which supported the development of antineoplastic drugs of IL-29.

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LI Liyun, LU Yuan, GE Chunlei, PENG Ronggang, LI Xinzhe, TAN Jing, CHEN Yongkang, CHEN Wei. Anti-Tumor Proliferation Activity of hIL-29 Variant[J]. Journal of Food Science and Biotechnology,2017,36(5):553-559.

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