Screening and Identification of β-Galactosidase-Producing Microorganism with Transgalactosylation Activity

doi:10.3969/j.issn.1673-1689.2017.06.006

Home > Archive>Volume 36, Issue 6, 2017 >598-603. DOI:10.3969/j.issn.1673-1689.2017.06.006

Screening and Identification of β-Galactosidase-Producing Microorganism with Transgalactosylation Activity
DOI:
                        10.3969/j.issn.1673-1689.2017.06.006
                    
CSTR:
                        [cstr]
                    
Author:
                        
                        
                    
Affiliation:
Clc Number:Q814.9
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

In order to obtain high enzyme activity of β-galactosidase，twenty-eight strains which produced β-galactosidase were screened from soil using lactose as inducer and X-gal as indicator.Four strains which have higher hydrolysis enzyme activity were screened by shake flask culturing. One strain B5582Y which have both of hydrolysis and transgalactosylation Activity was screened by HPLC，its hydrolysis of initial enzyme activity can reach upwards of 12 U/mL. And it was identified as Klebsiella michiganens is according to the cell morphology，physiological and biochemical characteristics，16SrDNA and rpoB functional gene sequence.

Reference

Cited by

Get Citation

ZHU Yunping, XU Liya, FAN Guangsen, WU Cheng, LI Xiuting. Screening and Identification of β-Galactosidase-Producing Microorganism with Transgalactosylation Activity[J]. Journal of Food Science and Biotechnology,2017,36(6):598-603.

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:
Revised:
Adopted:
Online: October 16,2017
Published:

Article QR Code

Copy Right：Editorial Board of Journal of Food Science and Biotechnology

Address：No. 1800, Lihu Avenue, Wuxi 214122, Jiangsu Province,China PostCode：214122

Phone：0510-85913526 E-mail：xbbjb@jiangnan.edu.cn

Supported by:Beijing E-Tiller Technology Development Co., Ltd.

Get Citation

Share

Article Metrics

History

Article QR Code

WeChat

Mobile website