Construction and Expression of Recombinant Superoxide Dismutase in Pichia pastoris

doi:10.3969/j.issn.1673-1689.2017.09.004

Home > Archive>Volume 36, Issue 9, 2017 >918-921. DOI:10.3969/j.issn.1673-1689.2017.09.004

Construction and Expression of Recombinant Superoxide Dismutase in Pichia pastoris
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                        10.3969/j.issn.1673-1689.2017.09.004
                    
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Superoxide dismutase （SOD） can be widely used in the field of food，cosmetic and medicine for its high capacity to scavenge free radicals. This paper reported a method of gene engineering attempting to solve the issue of SOD production. The gene of Cu，Zn-SOD with the yeast bias codon was firstly chemically synthesized and integrated into the genome of Pichia pastoris and the expression condition was optimized through orthogonal experiment. It was found that the maximum expression level of SOD was nearly three times greater than some reserach reported and the optimal temperature，methanol concentration and inoculation were 28 ℃，1.0% and 1.0% respectively. These results suggest that the problem of SOD production can be solved by improving the expression level of P.pastoris through the optimization of gene codon and fermentation conditions.

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ZHANG Hailing, LIU Chao, CHENG Yu, SUN Jinyu, ZHENG Yifeng, FU Ting, ZHAO Dan, LIU Shutao, RAO Pingfan. Construction and Expression of Recombinant Superoxide Dismutase in Pichia pastoris[J]. Journal of Food Science and Biotechnology,2017,36(9):918-921.

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Online: October 30,2017
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