Submission GuidelinesPublishing EthicsCopyright Statement Submitting Online
Home > Archive>Volume 36, Issue 9, 2017 >927-932. DOI:10.3969/j.issn.1673-1689.2017.09.006
PDF HTML XML Export Cite reminder
Quick Conversion From GPI-Positive to GPI-Negative Cells Using piggyBac Transposon System
CSTR:
[cstr]
Author:
Affiliation:

Clc Number:

Q786

Fund Project:

  • Article
    • |
  • Figures
    • |
  • Metrics
    • |
  • Reference
    • |
  • Related
    • |
  • Cited by
    • |
  • Materials
    • |
  • Comments
    Abstract:

    The biosynthesis of GPI is carried out in the endoplasmic reticulum（ER） through multiple steps and many genes are involved in the reactions. PIGK is one of the subunits of the catalytic domain of the GPI transmidase complex，responsible for the transfer of GPI moiety on protein. In this study，we generated a PIGK-knockout（KO） HEK 293 cell line，which showed no surface expression of GPI-anchored proteins（GPI-APs）. By mediation of piggyBac（PB） transposon system，we rescued the PIGK gene in PIGK-KO cells，which showed the recovery of the surface expression of GPI-APs. We successfully established a HEK 293 cell line，HEK293pB-PIGK，named G36，which can be converted from GPI-AP positive to negative cells by the introduction of PB transposase or FLP recombinase. Our system is useful to modulate surface proteins quickly and can be applied for both basic and applied researches.

    Reference
    Related
    Cited by
Get Citation

Matabaro Emmanuel, LIU Yishi, ZHANG Huijie, GAO Xiaodong, Morihisa Fujita. Quick Conversion From GPI-Positive to GPI-Negative Cells Using piggyBac Transposon System[J]. Journal of Food Science and Biotechnology,2017,36(9):927-932.

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:
  • Revised:
  • Adopted:
  • Online: October 30,2017
  • Published:
Article QR Code

Copy Right：Editorial Board of Journal of Food Science and Biotechnology

Address：No. 1800, Lihu Avenue, Wuxi 214122, Jiangsu Province,China  PostCode：214122

Phone：0510-85913526  E-mail：xbbjb@jiangnan.edu.cn

Supported by:Beijing E-Tiller Technology Development Co., Ltd.

WeChat

Mobile website