Enzyme Synthesis of AA-2G by Cyclodextrin Glycosyltransferase

doi:10.3969/j.issn.1673-1689.2018.01.005

Home > Archive>Volume 37, Issue 1, 2018 >27-32. DOI:10.3969/j.issn.1673-1689.2018.01.005

Enzyme Synthesis of AA-2G by Cyclodextrin Glycosyltransferase
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                        10.3969/j.issn.1673-1689.2018.01.005
                    
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The enzymatic synthesis condition of 2-O-alpha-D-glucopyranosyl-L-ascorbic acid（AA-2G） was studied in this dissertation. AA-2G was synthesized by using cyclodextrin glycosyltransferase（CGT-SL，support by Amano），and soluble starch and VC as the substrate. Under the initial condition，AA-2G production was 1.23 g/L. The best conditions for the synthesis of AA-2G：time 32 h，temperature 15 ℃，pH 4.0，concentration of VC 16 g/L，the proportion of VC and soluble starch is 2∶4，the concentration of CGT-SL 79 U/mL. Under these conditions，the final production of AA-2G was 6.23 g/L，and the transformation rate of VC reached 19.93%.

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SHAN Liyuan, LIU Long, LI Jianghua, DU Guocheng, CHEN Jian. Enzyme Synthesis of AA-2G by Cyclodextrin Glycosyltransferase[J]. Journal of Food Science and Biotechnology,2018,37(1):27-32.

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