Determination of Choline in Ganoderma lucidum and Its Spore Powder by HPLC-ELSD

doi:10.3969/j.issn.1673-1689.2018.01.006

Home > Archive>Volume 37, Issue 1, 2018 >33-37. DOI:10.3969/j.issn.1673-1689.2018.01.006

Determination of Choline in Ganoderma lucidum and Its Spore Powder by HPLC-ELSD
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                        10.3969/j.issn.1673-1689.2018.01.006
                    
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To establish an HPLC-ELSD method for determining the content of choline in Ganoderma lucidum spore powder. The experiment using Agilent company ZORBAX 300-SCX cation exchange chromatography column（4.6 mm×250 mm，5 μm）as the stationary phase，the column temperature was set at 30 ℃，the mobile phase was 0.3% acetonitrile - ammonium acetate solution（60∶40），with isocratic elution，the flow rate was 1.0 mL/min，the injection volume was 10 μL，the detector was evaporative light -scattering detector（ELSD），the drift tube temperature of the ELSD was set at 60 ℃，the spray mode was heated，and the purifying air pressure was 172.4 kPa. Research results show that the method precision，repeatability，stability and recovery rate are good.The choline showed a good linear relationship in the range of 6.25～200 μg/mL. The limit of detection was 6 μg/mL，and the limit of quantitation was 12 μg/mL.The results show tant it is a fast and efficient analysis method to determinate the content of choline in Ganoderma lucidum spore powder .

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WANG Yatao, LIU Yanfang, ZHANG Zhihua, ZHANG Zhong, ZHANG Jingsong, WANG Chenguang, ZHOU Shuai, TANG Qingjiu. Determination of Choline in Ganoderma lucidum and Its Spore Powder by HPLC-ELSD[J]. Journal of Food Science and Biotechnology,2018,37(1):33-37.

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Online: March 08,2018
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