Optimization of Fermentation Conditons for RGD-TRAIL Production by Recombinant E.coli Using Response Surface Method

doi:10.3969/j.issn.1673-1689.2018.03.009

Home > Archive>Volume 37, Issue 3, 2018 >283-288. DOI:10.3969/j.issn.1673-1689.2018.03.009

Optimization of Fermentation Conditons for RGD-TRAIL Production by Recombinant E.coli Using Response Surface Method
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                        10.3969/j.issn.1673-1689.2018.03.009
                    
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In order to enhance the production of RGD-TRAIL by recombinant E.coli，response surface methodology was applied to optimize the fermentation conditions. Based on the single factor experiments，the inoculation volume，pH value，the induction temperature and induction time were optimized by response surface optimization with 4 factors 3 levels using Box-Behnken experimental design. The optimum conditions for production of RGD-TRAILwere determined as follows：inoculation volume of 7.88%，pH 7.02，the induction time of 10.25 h，induction temperature of 22 ℃. Under the optimal fermentation conditions，the expression of RGD-TRAIL was 17.27%，which was close to the model prediction expression and 40.06% higher than that before optimization.

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CAO Jiaming, WEI Lijun, LIU Fusong. Optimization of Fermentation Conditons for RGD-TRAIL Production by Recombinant E. coli Using Response Surface Method[J]. Journal of Food Science and Biotechnology,2018,37(3):283-288.

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