Culture Optimization for Cellobiose Dehydrogenase Productionby Pycnoporus sanguineus SYBC-L10

doi:10.3969/j.issn.1673-1689.2018.03.012

Home > Archive>Volume 37, Issue 3, 2018 >303-308. DOI:10.3969/j.issn.1673-1689.2018.03.012

Culture Optimization for Cellobiose Dehydrogenase Productionby Pycnoporus sanguineus SYBC-L10
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                        10.3969/j.issn.1673-1689.2018.03.012
                    
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Effects of various parameters on cellobiose dehydrogenase（CDH） production by Pycnoporus sanguineus SYBC-L10. CDH was induced by cellulosic，and microcrystalline cellulose with high crystallinity supported the maximum CDH production. Though the optimization of fermentation medium composition，microcrystalline cellulose and glucose as the complex carbon source were add to the culture，the production of CDH reached 78±5.94 U/L，with 2 folds higher than that before optimization（39±4.78 U/L）.Though adding the soybean meal and（NH4）2HPO4 to the culture，the production of CDH reached 702±25.68 U/L，with 17 folds higher than that before optimization. Selecting the optimum carbon sources and nitrogen sources to get the optimum liquid medium recipe by the orthogonal test in four factors and four levers，The production of CDH reached 1245±35.78 U/L. The optimum fermentation medium consisted：microcrystalline cellulose 7 g/L，glucose 5 g/L，soybean meal 6 g/L，（NH4）2HPO4 2 g/L. The production of CDH reached 1 245±35.78 U/L.

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FENG Yuanyuan, TANG Mingyue, GUAN Zhengbing, CAI Yujie, LIAO Xiangru. Culture Optimization for Cellobiose Dehydrogenase Productionby Pycnoporus sanguineus SYBC-L10[J]. Journal of Food Science and Biotechnology,2018,37(3):303-308.

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