Construction of Escherichia coli Mutant Producing Novel Kdo2-Lipid A and Study of Its Immunological and Biochemical Activities

doi:10.3969/j.issn.1673-1689.2018.05.005

Home > Archive>Volume 37, Issue 5, 2018 >479-486. DOI:10.3969/j.issn.1673-1689.2018.05.005

Construction of Escherichia coli Mutant Producing Novel Kdo2-Lipid A and Study of Its Immunological and Biochemical Activities
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                        10.3969/j.issn.1673-1689.2018.05.005
                    
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Lipopolysaccharides（LPS） is the major component of the outer membrane of Gram-negative bacteria. It's composed of a hydrophobic Kdo2-lipid A and a hydrophilic polysaccharide chain，and can activate the innate immune system. Kdo2-lipid A is the bioactive centers of LPS，which has the potential to be developed as vaccines or vaccine adjuvants. In this study，we constructed a novel Kdo2-lipid A producing mutant BW003 by deleting waaC-waaF genes in the chromosome of a laboratory Escherichia coli strain. The Kdo2-lipid A structure of BW003 was confirmed by TLC and ESI/MS. Compared with wild type E. coli W3110 and LPS，BW003 and Kdo2-lipid A showed lower cytotoxicity when stimulating HEK-Blue hTLR4 cells and THP-1 cells. In addition，BW003 presented higher outer membrane permeability，sensitivity to antibiotics，hydrophobicity and auto-aggregation ability，which is safe and efficient for large-scale industrial fermentation and vaccine adjuvants development.

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WANG Biwen, LI Yanyan, WANG Xiaoyuan. Construction of Escherichia coli Mutant Producing Novel Kdo2-Lipid A and Study of Its Immunological and Biochemical Activities[J]. Journal of Food Science and Biotechnology,2018,37(5):479-486.

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