Rice Lipase Displayed on Pichia pastoris Surface Catalyzed Synthesis of Ethyl Acetate

doi:10.3969/j.issn.1673-1689.2018.05.013

Home > Archive>Volume 37, Issue 5, 2018 >542-546. DOI:10.3969/j.issn.1673-1689.2018.05.013

Rice Lipase Displayed on Pichia pastoris Surface Catalyzed Synthesis of Ethyl Acetate
DOI:
                        10.3969/j.issn.1673-1689.2018.05.013
                    
CSTR:
                        [cstr]
                    
Author:
                        
                        
                    
Affiliation:
Clc Number:TS201.2
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

In this study，the rice lipase（RL） was displayed on the surfaceof Pichia pastoris to catalyze synthesis of ethyl acetate in n-hexane，the influence of some factors such as the enzyme activity of rice lipase，substrate concentration，reaction temperature，reaction time，the amount of 3A molecular sieve on the synthesis of ethyl acetate was investigated. The study showed that，acetic acid / ethanol（mol/mol） ratio1.2∶1，reaction time of 4 h，reaction temperature of 40 ℃，the enzyme activityof rice lipase 15 750 U/g，the adding amount of 3A molecular sieve8 % in 15 mL n-hexane was the optimum condition . Under the condition，the reaction conversion of rice lipase catalyzed synthesis of ethyl acetate reached 90.92%.

Reference

Cited by

Get Citation

JIAO Ling, XIE Ding, WEN Li, JIANG Bo, CHEN Ping, ZHENG Ruina. Rice Lipase Displayed on Pichia pastoris Surface Catalyzed Synthesis of Ethyl Acetate[J]. Journal of Food Science and Biotechnology,2018,37(5):542-546.

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:
Revised:
Adopted:
Online: July 05,2018
Published:

Article QR Code

Copy Right：Editorial Board of Journal of Food Science and Biotechnology

Address：No. 1800, Lihu Avenue, Wuxi 214122, Jiangsu Province,China PostCode：214122

Phone：0510-85913526 E-mail：xbbjb@jiangnan.edu.cn

Supported by:Beijing E-Tiller Technology Development Co., Ltd.

Get Citation

Share

Article Metrics

History

Article QR Code

WeChat

Mobile website