Enzymatic Synthesis of S-methyl-L-cysteine Catalyzed by Tryptophan Synthase Genetically Engineered Microorganism

doi:10.3969/j.issn.1673-1689.2018.06.013

Home > Archive>Volume 37, Issue 6, 2018 >655-660. DOI:10.3969/j.issn.1673-1689.2018.06.013

Enzymatic Synthesis of S-methyl-L-cysteine Catalyzed by Tryptophan Synthase Genetically Engineered Microorganism
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                        10.3969/j.issn.1673-1689.2018.06.013
                    
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Tryptophan synthase genetically engineered microorganism was constructed by codon optimization in Escherichia coli. Preparation of S-methyl-L-cysteine from L-serine and methanthiol catalyzed by tryptophan synthase genetically engineered microorganism was investigated by response surface methodology and single-factor experiments. The optimal temperature and pH value were 39 ℃ and 8.5 respectively. The optimal substrate concentration of L-serine was 360 mmol/L. Under the optimal conditions of tryptophan synthase genetically engineered microorganism，the content of S-methyl-L-cysteine was 40.49 g/L after 20 h.

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XU Lisheng, GAO Guizhen, CAO Wengen, ZHAO Liang, ZHANG Xingtao, JIAO Qingcai, BAO Nina, SONG Man. Enzymatic Synthesis of S-methyl-L-cysteine Catalyzed by Tryptophan Synthase Genetically Engineered Microorganism[J]. Journal of Food Science and Biotechnology,2018,37(6):655-660.

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Online: September 27,2018
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