Studies of Microbiological Assay Folic Acid in Infant Milk

doi:10.3969/j.issn.1673-1689.2018.07.009

Home > Archive>Volume 37, Issue 7, 2018 >727-731. DOI:10.3969/j.issn.1673-1689.2018.07.009

Studies of Microbiological Assay Folic Acid in Infant Milk
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                        10.3969/j.issn.1673-1689.2018.07.009
                    
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According to the GB 5413.16 2010，GB/T 5009.211 2008 and Biostest different methods，On the same folic acid content in dairy products and for folic acid determination of standard addition recovery experiment was carried out at the same time. Results show that according to GB 5413.16 2010，in the preparation of standard test tube，using two concentration with phosphate buffer containing ascorbic acid dilution folic acid standard working liquid，liquid sample tests also diluted by ascorbic acid phosphate buffer，measured folic acid content is 40.45 μg/100 g，the recovery was 71%. But GB/T 5009.211 2008，using only a concentration of diluted folic acid standard working liquid，liquid sample tests also get diluted by water，measured the same samples of folic acid content is 70.5 μg/100 g，the recovery was 92.6%. Using Biostest folic acid detection kit measured the same samples of folic acid content is 73.71 μg/100 g，the recovery was 99%. Conclusion is not using ascorbic acid in phosphate buffer but diluted standard working liquid and liquid sample tests，test infant dairy folic acid content，the standard addition recovery rate is higher，test results are acceptable.

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SHANG Yinghui, LIU Xinjun, HUANG Hanchang, LAO Fengxue. Studies of Microbiological Assay Folic Acid in Infant Milk[J]. Journal of Food Science and Biotechnology,2018,37(7):727-731.

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Online: September 28,2018
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