Production of a New Xanthan Gum with Glycerol as Substrate

doi:10.3969/j.issn.1673-1689.2018.10.004

Home > Archive>Volume 37, Issue 10, 2018 >1028-1034. DOI:10.3969/j.issn.1673-1689.2018.10.004

Production of a New Xanthan Gum with Glycerol as Substrate
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                        10.3969/j.issn.1673-1689.2018.10.004
                    
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A mutant strain，which was named X. campestris WXLB-006 and could use glycerol for xanthan production，was obtained by adaptive evolution. RT-PCR results showed that the transcriptional level of genes related to glycerol metabolism（/i>glpF，glpK，glpD，and fbp ） in X. campestris WXLB-006 was higher than those of the parent strain，and the order of these four genes was glpD （8.53） > glpF （7.64） > glpK （6.61） > fbp （5.79）. With low glycerol concentration（40 g/L），adoption of variable aeration rate and stirring speed（0～24 h，0.5 vvm and 200 rpm；24～60 h，1.0 vvm and 400 rpm），and three-stage varied speed feeding glycerol strategy（24～34 h，3 g/L/h；34～44 h，2 g/L/h；44～54 h，1 g/L/h），biomass increased from 1.65 g/L to 1.94 g/L and xanthan yield from 17.8 g/L to 33.9 g/L，but fermentation time decreased from 120 h to 60 h. Importantly，xanthan gum produced from glycerol has the properties of low viscosity，high transparency，fast hydration rate，and increased viscosity after treated with -20 ℃ freeze-thaw cycles.

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WANG Zichao, ZHU Li, WU Jianrong, ZHENG Zhiyong, GAO Minjie, ZHAN Xiaobei. Production of a New Xanthan Gum with Glycerol as Substrate[J]. Journal of Food Science and Biotechnology,2018,37(10):1028-1034.

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Online: November 14,2018
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