Cloning，Expression and Characterization of a New Feruloyl Esterases from the Lactobacillus johnsonii Strain

doi:10.3969/j.issn.1673-1689.2018.11.002

Home > Archive>Volume 37, Issue 11, 2018 >1129-1134. DOI:10.3969/j.issn.1673-1689.2018.11.002

Cloning，Expression and Characterization of a New Feruloyl Esterases from the  Lactobacillus johnsonii  Strain
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                        10.3969/j.issn.1673-1689.2018.11.002
                    
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                        [cstr]
                    
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Ferulic acid is an important antioxidant，is a hot topic research in recent years. Ferulic acid esterase（FAEs） can hydrolyze the ester bond to release free ferulic acid. In this paper，according to the whole genome sequence of Lactobacillus johnsonii and the conserved sequence of ferulic acid esterase，the author designed a new encoding gene Lj0900 of ferulic acid esterase. By PCR amplification，enzyme connection，constructing the restructuring of the carrier，and successfully expressed in E. coli BL21. To study the preliminary properties of pure enzyme，crude FAEs was purified by nickel column. With the methyl ferulate as the substrate，discovered that the optimum temperature for the enzyme is 30 ℃，the optimum pH is 6.

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ZHANG Qiang, BAI Yajun, CAI Yujie, ZHENG Xiaohui. Cloning，Expression and Characterization of a New Feruloyl Esterases from the Lactobacillus johnsonii Strain[J]. Journal of Food Science and Biotechnology,2018,37(11):1129-1134.

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Online: December 25,2018
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