Effects of Dietary Acidolysis Products of Oxidized Konjac Glucomannan on Intestinal Microflora of Schizothorax prenanti

doi:10.3969/j.issn.1673-1689.2018.11.017

Home > Archive>Volume 37, Issue 11, 2018 >1225-1231. DOI:10.3969/j.issn.1673-1689.2018.11.017

Effects of Dietary Acidolysis Products of Oxidized Konjac Glucomannan on Intestinal Microflora of Schizothorax prenanti
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                        10.3969/j.issn.1673-1689.2018.11.017
                    
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To study the effects of dietary acidolysis products of oxidized konjac glucomannan（OKGM） on intestinal microflora of Schizothorax prenanti. A total of three hundred and sixty healthy fishes with average body weight of 81±2.64 g were randomly divided into 4 groups with 3 replicates and 30 fishes per replicate. The 4 groups were control group（basal diet）、LOKGM-0.8% group（basal diet + 0.8% LOKGM）、LOKGM-1.6% group（basal diet + 1.6% LOKGM）、LOKGM- 3.2% group（basal diet + 3.2% LOKGM），respectively. The experiment lasted for 75 d（15 d for adaptation and 60 d for test）. Diversity of posterior gut microflora was determined based on V3 variable regions of bacteria 16S rDNA by using denaturing gradient gel electrophoresis（DGGE） technique. The number of Aeromonas hydrophila and Bacillus in the intestinal was quantified by real-time quantitative PCR. The results showed as follows：compared with the control group，the diversity of posterior intestinal microflora was significantly increased in the LOKGM-1.6% and LOKGM-3.2% group（P<0.05），but the diversity of posterior intestinal microflora was not affected in the LOKGM-0.8% group（P>0.05）；the number of Aeromonas hydrophila in anterior and middle intestinal of Schizothorax prenanti was significantly decreased in the LOKGM-0.8% group（P<0.05），also the number of Bacillus in anterior intestinal was significantly increased（P<0.05）；the number of Aeromonas hydrophila in anterior，middle and posterior intestinal was all significantly decreased in LOKGM-1.6% group（P<0.05） and the number of Bacillus in middle and posterior intestinal was significantly increased（P<0.05）；the number of Aeromonas hydrophila in anterior and posterior intestinal was significantly decreased in LOKGM-3.2% group（P<0.05），besides，the number of Bacillus in anterior and middle intestinal was significantly increased（P<0.05）. In conclusion，acidolysis products of oxidized konjac glucomannan can improve the configuration of posterior intestinal microflora of Schizothorax prenanti and keep the fish healthy.

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YANG Min, CHEN Mingrui, WU Yinglong, YAN Qiuping, ZENG Liping. Effects of Dietary Acidolysis Products of Oxidized Konjac Glucomannan on Intestinal Microflora of Schizothorax prenanti[J]. Journal of Food Science and Biotechnology,2018,37(11):1225-1231.

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