Bioinformatics Analysis on the Contribution of N-Terminal Region of Xylanase EvXyn11TS to Its Thermostability

doi:10.3969/j.issn.1673-1689.2019.02.005

Home > Archive>Volume 38, Issue 2, 2019 >28-34. DOI:10.3969/j.issn.1673-1689.2019.02.005

Bioinformatics Analysis on the Contribution of N-Terminal Region of Xylanase EvXyn11TS to Its Thermostability
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                        10.3969/j.issn.1673-1689.2019.02.005
                    
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Effect of the N-terminus of a family 11 xylanase EvXyn11TS on its thermostability is systematically analyzed. Thirty xylanases that had the sequence homologies more than 55% aligned with EvXyn11TS and the known temperature characteristics are sought out from the GenBank database using the BLAST server. Then，the multiple alignment of 31 xylanase sequences is performed using the ClustalW2 program，and the phylogenetic tree is constructed using a MEGA 5.1 software. Eight representative thermophilic and mesophilic xylanases（including EvXyn11TS） are selected from the phylogenetic tree. Finally，the differences and similarities at the N-termini between EvXyn11TS and the other xylanases are analyzed using biology softwares. The analytical results indicate that the contents of amino acids favorable to thermostability，the β-strand A1 and the interactions between amino acids at the N-terminus of EvXyn11TS have certain contributions to its thermostability.

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ZANG Jia, LI Chuang, WANG Rui, WU Qin, WU Minchen. Bioinformatics Analysis on the Contribution of N-Terminal Region of Xylanase EvXyn11TS to Its Thermostability[J]. Journal of Food Science and Biotechnology,2019,38(2):28-34.

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