Metabolic Engineering of Saccharomyces cerevisiae for L-Malic Acid Production

doi:10.3969/j.issn.1673-1689.2019.02.011

Home > Archive>Volume 38, Issue 2, 2019 >72-80. DOI:10.3969/j.issn.1673-1689.2019.02.011

Metabolic Engineering of Saccharomyces cerevisiae for L-Malic Acid Production
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                        10.3969/j.issn.1673-1689.2019.02.011
                    
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With the purpose of elucidating the introduction of cytoplasmic reductive pathway in Saccharomyces cerevisiae on the production of L-malic acid， the reductive pathway for L-malic acid biosynthesis is constructed in S. cerevisiae by overexpressing genes Afpyc，Afmdh and Afmae from Aspergillus flavus. When gene Afpyc is kept in low level，the pyruvate titers of W003 are decreased 42% compared with control strain W001，respectively. To further introduced gene Afmdh in strain W003，the concentration of L-malic acid in W005 is up to 1.93 g/L，the DCW is increased by 350% and the pyruvate titers is decreased by 65.9% than that of the strain W004. Moreover，the malate efflux system is enhanced by introducting the tansporter gene Afmae and the L-malic acid titers of strain W006 is increased by 21.2%（2.34 g/L）. The concentrations of L-malic acid is increased from 2.34 g/L to 3.28 g/L with the initial OD600=2. It could be summarized that introducting the reductive pathway of overproducing L-malic acid strain A. flavus in S. cerevisiae is indeed producted L-malic acid，and this successful method provides a strategy to the metabolic engineering.

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CHEN Xiulai, WANG Yuancai, DONG Xiaoxiang, LUO Qiuling, LIU Jia, LIU Liming. Metabolic Engineering of Saccharomyces cerevisiae for L-Malic Acid Production[J]. Journal of Food Science and Biotechnology,2019,38(2):72-80.

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