Isolation，Purification，Structure Identification And Biological Activity of Polysaccharides From Stems And Leaves of "Nanshu 88"

doi:10.3969/j.issn.1673-1689.2019.02.015

Home > Archive>Volume 38, Issue 2, 2019 >101-110. DOI:10.3969/j.issn.1673-1689.2019.02.015

Isolation，Purification，Structure Identification And Biological Activity of Polysaccharides From Stems And Leaves of "Nanshu 88"
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                        10.3969/j.issn.1673-1689.2019.02.015
                    
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The "Nanshu 88" polysaccharide（N88P-1） is obtained from the dried stems and leaves of "Nanshu 88" by hot water extraction，ethanol precipitation，and then purified by DEAE-cellulose 52 column chromatography. The structure of "Nanshu 88" pure polysaccharide（N88P-1） is identification by Infrared spectroscopy（IR），High Performance Gel Penetration Chromatography（HPGPC），High Performance Liquid Phase Chromatographic（HPLC） and nuclear magnetic resonance spectra（1H NMR and 13C NMR）. At the same time，the antioxidant and immune regulation effects of N88P-1 are also carries out. The results show that N88P-1 has molecular weight of 14 328 D. The infrared spectra shows the characteristic peaks of O-H stretching vibration appears in 3 444.81 cm-1，and the characteristic peaks of C=O stretching vibration appears in 1 640.20 cm-1. 1H NMR spectra showed anomeric hydrogen signals at δ4.93~δ4.86. High performance liquid chromatography（HPLC） chromatography shows N88P-1 is composed of galactose and mannose with the ratio of 1∶1. The activity study shows the N88P-1 has ability to clear the ABTS + free radical in a certain range of concentration，and the IC50 value is 0.48 mg/mL. The DPPH - free radical scavenging activity of N88P-1 is positively correlated with concentration in the range of 0.25~3 mg/mL，the IC50 value is 0.96 mg/mL. When the concentration of N88P-1 is 3 mg/mL，the scavenging activity of ABTS + and DPPH - free radical both reach the highest value. The proliferation activity of the immune cells results show that the proliferation effect on B cells and T cells are very significant（P<0.01） in the concentration of 20~80 mg/mL；while the effect of macrophage proliferation was very significant（P<0.01） in the concentration of 10~40 mg/mL；It is worth noting that the proliferation effect of the three kinds of immune cells all reached the highest value in the concentration of 40 mg/mL.

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LIU Lu, HOU Yiling, WANG Mei, ZHAO Daqun, TAN Chunmei, LIU Xingli, HOU Wanru, DING Xiang. Isolation，Purification，Structure Identification And Biological Activity of Polysaccharides From Stems And Leaves of "Nanshu 88"[J]. Journal of Food Science and Biotechnology,2019,38(2):101-110.

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Online: March 28,2019
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