Improving the Activity and Catalytic Efficiency of Formate Dehydrogenase（CbFDH） by Site-Directed Mutagenesis

doi:10.3969/j.issn.1673-1689.2019.03.001

Home > Archive>Volume 38, Issue 3, 2019 >1-9. DOI:10.3969/j.issn.1673-1689.2019.03.001

Improving the Activity and Catalytic Efficiency of Formate Dehydrogenase（CbFDH） by Site-Directed Mutagenesis
DOI:
                        10.3969/j.issn.1673-1689.2019.03.001
                    
CSTR:
                        [cstr]
                    
Author:
                        
                        
                    
Affiliation:
Clc Number:Q786
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

Formate dehydrogenase（FDH） is an industrial enzyme widely used for NADH regeneration. However，the low activity and low catalytic efficiency are flaws of native FDH. In this study，amino acid sequences alignment of FDH from different sources was conducted. The result showed that the 93-site amino acid of Candida boidinii FDH（CbFDH） was valine（V），while that of others were isoleucine（I）. Moreover，according to the hydrophobicity，four mutant enzymes V93L，V93I，V93A and V93G were constructed. The enzymatic properties showed that the activity of mutants was improved as the enhancement of 93-site amino acids hydrophobicity，except mutant V93L. And the activity and catalytic efficiency（for NAD+） of V93I were increased by 22.6% and 133%，respectively. Mutant V93I and L-leucine dehydrogenase were used to synthesize L-norvaline by asymmetric reduction of α-ketovaleric acid. The results showed that the conversion efficiency of α-ketovaleric acid was improved with mutant V93I.

Reference

Cited by

Get Citation

ZHENG Junxian, WANG Ziyuan, YANG Taowei, ZHANG Xian, XU Meijuan, RAO Zhiming. Improving the Activity and Catalytic Efficiency of Formate Dehydrogenase（CbFDH） by Site-Directed Mutagenesis[J]. Journal of Food Science and Biotechnology,2019,38(3):1-9.

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:
Revised:
Adopted:
Online: June 21,2019
Published:

Article QR Code

Copy Right：Editorial Board of Journal of Food Science and Biotechnology

Address：No. 1800, Lihu Avenue, Wuxi 214122, Jiangsu Province,China PostCode：214122

Phone：0510-85913526 E-mail：xbbjb@jiangnan.edu.cn

Supported by:Beijing E-Tiller Technology Development Co., Ltd.

Get Citation

Share

Article Metrics

History

Article QR Code

WeChat

Mobile website