Inhibition Effect of Sodium Lactate on Biofilm Formation by Listeria monocytogenes

doi:10.3969/j.issn.1673-1689.2019.03.003

Home > Archive>Volume 38, Issue 3, 2019 >17-25. DOI:10.3969/j.issn.1673-1689.2019.03.003

Inhibition Effect of Sodium Lactate on Biofilm Formation by Listeria monocytogenes
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                        10.3969/j.issn.1673-1689.2019.03.003
                    
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The objective of this study was to investigate the inhibition effet of sodium lactate（NaL） on biofilm formation by Listeria monocytogenes. First of all，the crystal violet staining assay was used to evaluate the inhibition effect of NaL at different concentrations（0%，2.5%，5%，10% and 20%） on the biofilm formation of L. monocytogenes. Furthermore，the effets of NaL on its biofilm structure，polysaccharides，extracelluar proteins，cell activity and membrane integrity of L. monocytogenes biofilm cells were also investigated. Finally，the detection of the expression level of the biofilm-associated genes motB，mogR，degU，flgE，dnaK，prfA and sigB was performed by using quantitative real-time PCR（qRT-PCR）. With the increase of the NaL concentration（2.5~20%），biofilm formation of L. monocytogenes was inhibited significantly（p<0.05） and the inhibition rate was 8.34 %，32.2 %，46.6 % and 55.2 %，respectively. Results of microscopy analysis showed that after 5 % NaL treatment，the structure of L. monocytogenes biofilm was forming instead of small clumps with reduction of thickness，and a significant decrease（p<0.05） was determined in total production of polysaccharides and extracelluar proteins of L. monocytogenes biofilm cells. In addition，5 % NaL inhibited the cell activity and membrane integrity of L. monocytogenes biofilm cells to suppress the development of new biofilms. qRT-PCR results revealed that NaL could downregulate biofilm-associated genes in L. monocytogenes and therefore to inhibit its biofilm formation. In conclusion，this study could provide a scientific basis for the effective inhibition of NaL on L. monocytogenes biofilm formation.

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FU Jiaojiao, WANG Xu, LIU Haiquan, SUN Xiaohong, XIE Jing, PAN Yingjie, ZHAO Yong. Inhibition Effect of Sodium Lactate on Biofilm Formation by Listeria monocytogenes[J]. Journal of Food Science and Biotechnology,2019,38(3):17-25.

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Online: June 21,2019
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