Improve the Synthesis of L-Phenylglycine by Increasing the Intracellular Coenzyme Level

doi:10.3969/j.issn.1673-1689.2019.03.007

Home > Archive>Volume 38, Issue 3, 2019 >46-53. DOI:10.3969/j.issn.1673-1689.2019.03.007

Improve the Synthesis of L-Phenylglycine by Increasing the Intracellular Coenzyme Level
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                        10.3969/j.issn.1673-1689.2019.03.007
                    
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Coenzyme NAD（H） is necessary for whole cell synthesis of L-phenylglycine，so intracellular NAD（H） level is critical for L-phenylglycine synthesis. In this study，the co-expression of the genes pncB and nadE encoding of nicotinic acid phosphoribosyltransferase and NAD+ synthase separately was conducted to increase intracellular NAD（H） level so as to accelerate the whole-cell transformation. The results showed that the NAD（H） level in whole cell was increased by 2.35-fold when pncB and nadE were co-expressed，and it was further increased by 2.42-fold with the addition of 20 mg/L nicotinic acid（NA）. Significantly，the whole cell catalytic activity and L- phenylglycine yield were increased by 94% and 36.6%，respectively，indicating that the transformation efficiency can be effectively improved by the increase of intracellular coenzyme levels in the coenzyme- dependent whole cell transformation systems.

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LIU Qiaoli, YANG Taowei, ZHOU Junping, XU Meijuan, ZHANG Xian, RAO Zhiming. Improve the Synthesis of L-Phenylglycine by Increasing the Intracellular Coenzyme Level[J]. Journal of Food Science and Biotechnology,2019,38(3):46-53.

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Online: June 21,2019
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