Study of Mechanisms of Extract Fraction 3 from Antrodia camphorate Attenuating TGF-β1-Induced Liver Fibrosis

doi:10.3969/j.issn.1673-1689.2019.03.008

Home > Archive>Volume 38, Issue 3, 2019 >54-60. DOI:10.3969/j.issn.1673-1689.2019.03.008

Study of Mechanisms of Extract Fraction 3 from Antrodia camphorate Attenuating TGF-β1-Induced Liver Fibrosis
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                        10.3969/j.issn.1673-1689.2019.03.008
                    
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To investigate the effects of hexane extract fraction 3（Fr.3） from Antrodia camphorate on hepatic stellate cells（HSCs） activation induced by TGF-β1，as well as mechanisms of its anti-fibrotic action in vitro. We established a cell model of liver fibrosis by using CFSC-8B stimulated by TGF-β1. Cell proliferation，cell migration，extracellular matrix（ECM） production and protein expression in the TGF-β1 signaling pathways were detected by the MTT，WST-1 assay，cell migration assay，qRT-PCR and western blotting analyses. The IC50 value of Fr.3 was 54.56 μg/mL and we found that less than 20 μg/mL of Fr.3 might be safe for CSFC-8B cells. Fr.3（< 20 μg/mL） significantly inhibited CFSC-8B cell proliferation and migration stimulated by TGF-β1. Fr.3 also downregulated α-SMA，Col1，Col3 and Fn mRNA expression. Mechanistically，Fr.3 blocked TGF-β1 induced protein expression of α-SMA，Col1，p-Smad2，p-ERK，p-P38 and p-AKT. Fr.3 from A. camphorata exhibits anti-hepatic fibrosis activity through negatively modulating TGF-β1/Smad/PI3K/MAPK signaling pathways in CFSC-8B cells.

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SUN Qing, GENG Yan, DU Yan, XU Zhenghong. Study of Mechanisms of Extract Fraction 3 from Antrodia camphorate Attenuating TGF-β1-Induced Liver Fibrosis[J]. Journal of Food Science and Biotechnology,2019,38(3):54-60.

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Online: June 21,2019
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