Role of 6-Phosphogluconate Dehydrogenase in Lipid Accumulation of Oleaginous Bacteria Rhodococcus opacus PD630

doi:10.3969/j.issn.1673-1689.2019.03.009

Home > Archive>Volume 38, Issue 3, 2019 >61-68. DOI:10.3969/j.issn.1673-1689.2019.03.009

Role of 6-Phosphogluconate Dehydrogenase in Lipid Accumulation of Oleaginous Bacteria Rhodococcus opacus PD630
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                        10.3969/j.issn.1673-1689.2019.03.009
                    
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The process of lipid biosynthesis in oleaginous microorganisms is basically clear：both the reducing power NADPH and the building block acetyl-CoA are required by fatty acid synthase（FAS） to synthesize fatty acids. In oleaginous microorganisms，the main enzymes involved in the provision of reducing power NADPH are malic enzyme（ME），glucose-6-phosphate dehydrogenase（G6PDH），6-phosphogluconate dehydrogenase（6PGDH），and isocitrate dehydrogenase（ICDH）. In this study，the genes coding for 6-phosphogluconate dehydrogenase（gnd） from the oxidative pentose phosphate pathway（oxPPP） were over-expressed in the oleaginous bacteria Rhodococcus opacus PD630 to analyze its effect on lipid accumulation. The results showed that over-expression of gnd increased the content of lipid（w/w） by 20%~30% compared with the control strain. Meanwhile，the activities and the mRNA levels of 6PGDH in the overexpressing strains were higher than that of the control. Therefore，it was concluded that 6PGDH enhanced the lipid accumulation through promoting the generation of NADPH in the oleaginous bacteria R. opacus PD630.

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ZHANG Dongdong, ZHAO Lina, ZAN Xinyi, TANG Xin, SONG Yuanda. Role of 6-Phosphogluconate Dehydrogenase in Lipid Accumulation of Oleaginous Bacteria Rhodococcus opacus PD630[J]. Journal of Food Science and Biotechnology,2019,38(3):61-68.

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