Screening of Butanol-Tolerant Escherichia coli Strain by Global Transcription Machinery Engineering and Its Characterization

doi:10.3969/j.issn.1673-1689.2019.03.011

Home > Archive>Volume 38, Issue 3, 2019 >76-82. DOI:10.3969/j.issn.1673-1689.2019.03.011

Screening of Butanol-Tolerant Escherichia coli Strain by Global Transcription Machinery Engineering and Its Characterization
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                        10.3969/j.issn.1673-1689.2019.03.011
                    
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In this study，we employed a fast and efficient approach，Global Transcription Machinery Engineering（gTME），to improve the butanol tolerance of Escherichia coli. The results show that one mutant E. coli JM109/pHACMB8 harboring rpoD mutant B8 could tolerate 2%（v/v） butanol. Further studies showed that the mutant B8 also exhibited high tolerance to other organic solvents such as 3%（v/v） isopropanol，8%（v/v） ethanol，4%（v/v） cyclohexane and 0.3%（v/v） toluene. To further reveal the tolerance mechanism of the mutant，we investigated the physiological characteristics of both /i> E. coli JM109/pHACMWT and the mutant B8. The results show that the intracellular butanol concentration of mutant B8 was 55% lower and has improved acid tolerance compared with WT. In addition，Mg2+ was proved to have a beneficial effect on the butanol tolerance of B8 mutant. Our study provides experimental data and theoretical basis for constructing OST bacteria for industrial applications.

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SI Haiming, HAN Ruizhi, XU Guochao, DONG Jinjun, NI Ye. Screening of Butanol-Tolerant Escherichia coli Strain by Global Transcription Machinery Engineering and Its Characterization[J]. Journal of Food Science and Biotechnology,2019,38(3):76-82.

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Online: June 21,2019
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