Enhancement of L-Citrulline Production by Promoter Modification of argGH in Corynebacerium crenatum

doi:10.3969/j.issn.1673-1689.2019.03.012

Home > Archive>Volume 38, Issue 3, 2019 >83-90. DOI:10.3969/j.issn.1673-1689.2019.03.012

Enhancement of L-Citrulline Production by Promoter Modification of  argGH in  Corynebacerium crenatum
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                        10.3969/j.issn.1673-1689.2019.03.012
                    
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L-citrulline isan importantintermediate metabolite inurea cycle. The enzymes encoded by gene argGH catalyze thereaction of L-citrullineto L-arginineresults in difficulty of L-citrulline accumulation via microbial metabolism. In this study，promoter P-argG wasreplaced by a weak promoter P- dapAB6 to down-regulate argGH expression. The recombinant strain Corynebacterium crenatum H-7-P dapAB6： argGH showed lower argGH expression level，with ASS（argininosuccinate synthase，encoding by argG） and ASL（argininosuccinase，encoding by argH） activities reducing by 91.80% and 55.35%，respectively，compared with the parent strain. Via fermentation in shake flasks，the L-citrulline titer，yield and productivity of Corynebacterium crenatum H-7-P dapAB6：argGH were 33.85 g/L，0.25 g/g and 0.34 g/（L·h），respectively，which were 4.91，5.00 and 4.86-fold of those obtained from the parentstrain. These results indicatedthat，by promoter engineeringapproach，the preliminary construction of L-citrulline biosynthesis pathway was achieved.

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ZHANG Weiwei, CHEN Ziwei, ZHANG Xiaojuan, DOU Wenfang, ZHANG Xiaomei, GENG Yan, XU Zhenghong. Enhancement of L-Citrulline Production by Promoter Modification of argGH in Corynebacerium crenatum[J]. Journal of Food Science and Biotechnology,2019,38(3):83-90.

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