Effects of Nitrogen Source on Biochemical Composition，Cell Ultrastructure and Production of Nannocloropsis oculata

doi:10.3969/j.issn.1673-1689.2019.03.014

Home > Archive>Volume 38, Issue 3, 2019 >97-102. DOI:10.3969/j.issn.1673-1689.2019.03.014

Effects of Nitrogen Source on Biochemical Composition，Cell Ultrastructure and Production of Nannocloropsis oculata
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                        10.3969/j.issn.1673-1689.2019.03.014
                    
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In order to increase the biomass and productivity of Nannochloropsis oculata，the effects of three kinds of nitrogen sources (ammonium chloride，ammonium acetate and sodium nitrate) on physiology-biochemistry and production of N.oculata were compared in this research paper. The total cellular protein content of ammonium groups (ammonium chloride and ammonium acetate) were higher than that of sodium nitrate group，while cellular chlorophyll a content showed the opposite result； the total cellular oil content of ammonium acetate group was the highest，being 13.5%； the total cellular carbohydrate content of nitrate sodium group was the highest，being 16.5%. The polar lipid to neutral lipid ratio and the relative content of EPA of sodium nitrate group were the highest，being 5.9 and 26.6% respectively. The microalgal cell size of the ammonium groups were larger than that of sodium nitrate group； the number，relative volume and thylakoid density of chloroplast of sodium nitrate group were higher than those of ammonium groups； only the cells of ammonium acetate group contained oil bodies. The results showed that the biomass and productivity of ammonium acetate group were the highest，being 873 mg/L and 25.3 mg/(L·d) respectively.

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WANG Xin, WANG Yuxian, ZHAO Ben, YANG Hailin. Effects of Nitrogen Source on Biochemical Composition，Cell Ultrastructure and Production of Nannocloropsis oculata[J]. Journal of Food Science and Biotechnology,2019,38(3):97-102.

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