Ameliorative Effects of Extract Fraction 8 from Cephalosporium sinensis on Insulin Resistance of HepG-2 Cells

doi:10.3969/j.issn.1673-1689.2019.03.016

Home > Archive>Volume 38, Issue 3, 2019 >111-117. DOI:10.3969/j.issn.1673-1689.2019.03.016

Ameliorative Effects of Extract Fraction 8 from Cephalosporium sinensis on Insulin Resistance of HepG-2 Cells
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                        10.3969/j.issn.1673-1689.2019.03.016
                    
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To investigate the ameliorative effects of extract fraction 8（Fr.8） from Cephalosporium sinensis on insulin resistance of HepG-2 cells，in this study，a stable insulin resistance cell model in vitro was established to detect the glucose consumption，glucose uptake，relative mRNA expression and protein expression of Fr.8 on HepG-2/IR cells.The results showed that 5 μmol/L insulin was the optimal concentration to induce HepG-2 cells become HepG-2/IR cells. In the range of non-cytotoxic，Fr.8 could distinctly promote glucose consumption and uptake in HepG-2 / IR cells.Fr.8（>25 μg/mL） significantly upregulated IR，IRS1，IRS2，Glut2，AMPKα mRNA expression and obviously increased IR，IRS，Glut2，p-AMPKα and p-AKT protein expression.Therefore，we provide theoretical basis to demonstrate Fr.8 from C. sinensis ameliorated insulin resistance of HepG-2 cells，which in part，the mechanism may be associated with insulin receptor and substrate，glucose transporter protein 2，the activated protein of p-AMPKα and p-AKT.

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HAN Yifeng, GENG Yan, MENG Zhaomin, DU Yan, XU Hongyu, SHI Jinsong, XU Zhenghong. Ameliorative Effects of Extract Fraction 8 from Cephalosporium sinensis on Insulin Resistance of HepG-2 Cells[J]. Journal of Food Science and Biotechnology,2019,38(3):111-117.

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